It is thus appropriate to modify as follows the existing Requirements for. Rabies Vaccine for Human Use. General considerations (page 58). Replace the. Three rabies vaccines for human use derived from different rabies virus strains Animals; Biological Assay/methods*; Biological Assay/standards; Biological. BIOLOGICAL ASSAY OF RABIES ANTISERUM: The potency of rabies antiserum is determined by comparing a lethal intracerebral dose of.

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Dephtheria anti-toxin is preparation containing antitoxic globulins that they have the power of specifically neutralizing the toxin formed by Cornebacterium diphtheria.

Measure all the responses are calculated the result of the assay by std statistical method.

Ratio between two dose of test and two dose of standard should be equal This ratio kept constant through out the assay. Oestrogen progesterone and prolactin — responsible for production of milk by memory gland but milk ejection require oxytocin. Measure all the response and calculate the result of the assay by standard statistical method. Dilute 25ml of basal medium stock solution with equal volume of H2O sterile in autoclave.

Share to Twitter Share to Facebook. Rabies can be transmitted transplacentally Transplants in human- possible Human-to-human: Sequential Steps of Rabies Transmission Virus lifestyle: Dose should be added at regular interval[minutes] Slide Centrifuge decant of supernatant liquid [repeat 3 times].


P otency of rabies vaccine is determined by comparing a lethal intra-cerebral dose of a pf virus with the dose lf the standard preparation of rabies vaccines necessary to give for same protection.

Then they are incubated or about 24 hours at about and the diameter or areas of the circular inhibition zones are measured. In order to view it, please contact the author of the presentation. Thanks for shearing about this I thinks its very hopeful post and very important post for us. P response Dose should cause decrease in B.

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Incubation at c for hrs. Any suitable strain rabies virus of known potency, such as the CVS strain may be used.

I am very happy to your post about on. HCl [to PH 6. Centrifuge decant supernatant liquid. Oxytocin facilitates the contraction of uterus. The test toxin is allowed to stand for some months off being used for the assay of samples of antitoxin.

In selecting a toxin for use as the test toxin determine the following: Dose should be added at regular interval[minutes]. Incubation time days. Inject a dose of 0.

The volume of solution added to each cylinder or cavity must be uniform and sufficient to fill the holes. Upload from Desktop Single File Upload.

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Viral disease – cause acute encephalitis inflammation of vacccine brain in warm-blooded animals. Consciousness initially spared Two forms can overlap or progress from one to the other Coma after one week of neuro symptoms with death a few days after. White mice weight 11gg. Internal between two injection between minutes depending on the rate at which B. Maintain its sterility by the addition of toluene or other antimicrobial preservative which does not cause a rapid decline in specific toxicity.


Potency is determined by international reference Slide Acid digested casein solution. Dilute the test toxin with saline solution so that 1. Potency is determined by international reference. Standard preparation Test preparation To triplicate test tubes 0ml 0.

PHARMACOLOGY: Biological Assay of Diphtheria Antitoxin

Similarly record the contraction of test preparation as standard. The potency of diphtheria anti-toxin is determined by comparing the dose necessary to protect guinea-pig or rabbits against the erythrogenic effect of a fixed dose of the standard preparation of diphtheria anti toxin necessary to give the same protection.

Allow the mixtures to stand at room temperature, protected from light, for 15 to 60 minutes.

Tetanus anti-toxin is a preparation containing antitoxic globulins that have the power of specifically neutralizing the toxin formed by clostridium tetani. It is obtained by fractionation from the serum of horses or other mammals that have been immunized against diphtheria toxin.

Suspended cells in 10ml of sterile suspension medium.

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